Since cosmetics are essential for human existence, it is crucial to guarantee their efficacy and safety. The current chapter focuses on cosmetic ingredients like preservatives, fragrances, and colorants and the involvement of 3D skin model systems in their safety evaluation. At the beginning of the chapter, we briefly understand different types of 3D skin models currently available and the advantages of using these systems over current in vitro assays when it comes to toxicity testing for cosmetic products. Because the primary route of exposure for cosmetic products is dermal, we then go on to give general regulatory guidelines for dermal toxicity testing and recommendation for 3D skin models in dermal testing. The chapter then delves into details of each ingredient mentioned above and reports the usage of 3D skin models in the safety testing of these ingredients.