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Evaluation of unsupervised learning algorithms for the classification of behavior from pose estimation data
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).ORCID iD: 0000-0002-8902-3238
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Number of Authors: 52025 (English)In: Patterns, E-ISSN 2666-3899, Vol. 6, no 5, article id 101237Article in journal (Refereed) Published
Abstract [en]

Analyzing animal behavior is crucial for decoding brain function, modeling neurological disorders, and assessing therapeutics. Recent advances in pose-estimation tools like DeepLabCut and SLEAP have revolutionized behavioral analysis by enabling precise tracking of animal body movements. However, these tools do not automate behavioral classification. Unsupervised learning algorithms address this gap by identifying clusters of recurring behavioral motifs from pose-tracking data without requiring pre-labeled datasets, reducing observer bias and uncovering novel patterns. This study compares four recent unsupervised learning algorithms—B-SOiD, BFA, VAME, and Keypoint-MoSeq—analyzing their methodological approaches, clustering efficiency, and ability to produce meaningful behavioral classifications. By offering a qualitative and quantitative evaluation, this paper aims to aid researchers in selecting the most suitable tool for their specific research needs.

Place, publisher, year, edition, pages
2025. Vol. 6, no 5, article id 101237
Keywords [en]
behavioral classification, neuroethology, neuroscience, unsupervised learning
National Category
Behavioral Sciences Biology Artificial Intelligence
Identifiers
URN: urn:nbn:se:su:diva-243064DOI: 10.1016/j.patter.2025.101237ISI: 001493722000005Scopus ID: 2-s2.0-105003103402OAI: oai:DiVA.org:su-243064DiVA, id: diva2:1957310
Available from: 2025-05-09 Created: 2025-05-09 Last updated: 2025-09-18Bibliographically approved

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Mlost, JakubCosta, Ana RitaPollak Dorocic, Iskra

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